Methane emissions from anoxic freshwater contribute to global warming. This impact is set to worsen with increased temperatures and nutrient inputs making more anoxic freshwater, increasing methane emission rates. These two projects, which were inspired by work carried out at CEM, will investigate the growth of ostensibly aerobic methane-consuming bacteria in such anoxic environments. Genomic analysis indicates that certain methanotrophic bacteria may be able to exploit extracellular electron transport to survive without oxygen by using metals as electron acceptors. By experimenting on pure cultures and enrichment cultures of methanotrophic bacteria we will test whether this is possible. Such knowledge will help us better understand carbon cycling, mitigate methane emissions, and put methanotrophic bacteria to better use in green biotechnology.